THE EFFECT OF SULPHUR ON...

Tom XXV, Rośliny Oleiste 2004

THE EFFECT OF SULPHUR ON GLUCOSINOLATE CONTENT IN SEEDLINGS OF SPRING OILSEED RAPE STAR

Piotr Mirosław Szulc, Lucyna Drozdowska

Akademia Techniczno-Rolnicza w Bydgoszczy, Wydział Rolniczy, Katedra Fizjologii Roślin

Key words: spring oilseed rape, seedlings, in vitro culture, sulphur, indolyl glucosinolates, alkenyl glucosinolates

The effect of sulphur on the content and turnover of glucosinolates in ontogenesis of spring oilseed rape was studied in an experiment carried out under controlled in vitro cultures.

Three-day-old germinating seeds and 6 to 21-day-old oilseed rape seedlings were studied. They were grown on the modified Murashige and Skoog medium (1962) without sulphur (control) and containing from 172 to 767 mg S-SO 4 dm" 3 . Germinated seeds and seedlings were 1 yophylised and homogenised and than analysed for glucosinolate content with high performance liquid chromato-graphy (HPLC) according to EN ISO 9167-1.

Main glucosinolates in three-day-old seedlings were alkenyl glucosinolates (mainly progoitrin), whereas in 9-21 day-old seedlings indolyl glucosinolates (mainly neoglucobrassicin) predominated.

Lack of sulphur as well as its deficit in the medium in early steps of ontogenesis accelerated catabolism of glucosinolates. It suggested that products of hydrolysis of those compounds can be used as a source of sulphur by the developing embryo at a heterotrophic phase of germination.

Mailing address:

Szulc Piotr
Skrytka pocztowa 75
85-959 Bydgoszcz 2
Poland

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